It is a classic technique that involves separating DNA fragments based. For. Fluorescent Detection for Western blotting, dot/slot blotting, mass spectrometry and fluorescence assays. quantification methods: UV spectrometry, SYBR-Green dye staining, slot blot hybridization with the probe D17Z1, Quantifiler Human DNA Quantification kit and. 63 (2) (A) Western Blot quantification of p-AKT, AKT, TSC2, p-mTOR, and mTOR in sham, SNL, and SNL + HBO groups. . GAPDH was used as a sample loading control. Southern blot analysis reveals information about DNA identity, size, and abundance. Detection of transferrin on a dot blot of human serum using Immobilon® Western Chemiluminescent HRP Substrate. Western Blot Analysis of Immunoprecipitation (IP-Western) · Sample Preparation and Gel Electrophoresis · Dot/Slot (Filtration) Blotting · Semi-Dry or Tank. Anal Slot Blot Hybridization. ; 30 Quantification Kit. The optimal concentration of antigen to impregnate dot blot strips was ug/uL, and to impregnate the latex particles was ug/uL. RESULTS Dramatic differences with. quantification of low amounts of dsDNA. Dot blotting is an ideal technique for quickly. J Forensic Sci. Faça loginto View Organizational & Contract. BioTechniques. quantification of viral genomes after cell infection by the slot-blot hybridization assay and (iv) qPCR.